Anticorps Polyclonal de lapin anti-BDNF

• Phare
• Validé par KD/KO

BDNF Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (1)

N° de cat : 25699-1-AP

Synonymes

Abrineurin, BDNF

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Galerie de données de validation

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  WB analysis of fetal human brain using 25699-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25699-1-AP (BDNF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 25699-1-AP

  WB result of BDNF antibody (25699-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDNF transfected U-251 cells.

• 

  WB analysis of rat brain using 25699-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25699-1-AP (BDNF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25699-1-AP (BDNF Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25699-1-AP (BDNF Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25699-1-AP (BDNF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25699-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25699-1-AP (BDNF Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules U-251, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 10 publications below
• IF: See 3 publications below

Informations sur le produit

25699-1-AP cible BDNF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris, Macaque
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BDNF Protéine recombinante Ag22489
• Nom complet: brain-derived neurotrophic factor
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC029795
• Symbole du gène: BDNF
• Identification du gène (NCBI): 627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publications

Species	Application	Title
mouse	IHC	J Neurosci; Early BDNF Treatment Ameliorates Cell Loss in the Entorhinal Cortex of APP Transgenic Mice.; Authors - Nagahara Alan H AH
mouse	WB	Neuropsychopharmacology; CPEB3-dowregulated Nr3c1 mRNA translation confers resilience to developing posttraumatic stress disorder-like behavior in fear-conditioned mice.; Authors - Wen-Hsin Lu
mouse	WB	Cell Mol Life Sci; Furin promotes dendritic morphogenesis and learning and memory in transgenic mice.; Authors - Binglin Zhu
	IHC	J Neurotrauma; Bone Marrow Stromal Cell Intraspinal Transplants Fail to Improve Motor Outcomes in a Severe Model of Spinal Cord Injury.; Authors - John H Brock
mouse	WB	Exp Neurol; Neuroprotective and neuro-rehabilitative effects of acute purinergic receptor P2X4 (P2X4R) blockade after ischemic stroke.; Authors - Pranay Srivastava
mouse	WB	Front Pharmacol; Danshen-Chuanxiong-Honghua Ameliorates Cerebral Impairment and Improves Spatial Cognitive Deficits after Transient Focal Ischemia and Identification of Active Compounds.; Authors - Xianhua Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Armelle (Verified Customer) (09-03-2019)

This antibody worked very well. We were able to quantify expression levels in our area of interest.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: bain slice fom the ventrolateral hypothalamus

BDNF Antibody Immunofluorescence, validation (1/500 dilution) in bain slice fom the ventrolateral hypothalamus (Cat no:25699-1-AP)

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