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Anticorps Polyclonal de lapin anti-BAIAP2L1

BAIAP2L1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 25692-1-AP

Synonymes

BAIAP2L1, IRTKS

Galerie de données de validation

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  • Western Blot (WB) analysis of HeLa cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of HeLa using 25692-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of K-562 using 25692-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of SGC-7901 using 25692-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Applications testées

Résultats positifs en WBcellules HeLa, cellules K-562, cellules SGC-7901
Résultats positifs en IHCtissu de tumeur ovarienne humain, tissu de cancer de l'estomac humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

25692-1-AP cible BAIAP2L1 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène BAIAP2L1 Protéine recombinante Ag22499
Nom complet BAI1-associated protein 2-like 1
Masse moléculaire calculée 511 aa, 57 kDa
Poids moléculaire observé 57 kDa
Numéro d’acquisition GenBankBC013888
Symbole du gène BAIAP2L1
Identification du gène (NCBI) 55971
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAIAP2L1, also known as IRTKS, is a 511-amino acid protein containing an IRSp53/MIM homology domain (IMD) that bundles actin filaments and interacts with the small GTPase Rac (PMID: 17430976; 14752106). In addition to the IMD domain, BAIAP2L1 also contains an SH3 domain and WW domain interaction motif. BAIAP2L1 is widely distributed and is an INS receptor substrate. It has a role in actin remodeling and membrane protrusion.

Protocole

Product Specific Protocols
WB protocol for BAIAP2L1 antibody 25692-1-APDownload protocol
IHC protocol for BAIAP2L1 antibody 25692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects both human and mouse versions of the protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T and overexpression
BAIAP2L1 Antibody Western Blot, validation (1:1000 dilution) in 293T and overexpression (Cat no:25692-1-AP)