IRSp53 Polyclonal antibody

IRSp53 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 11087-2-AP

Synonyms

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSp53, IRSp53/58, Protein BAP2

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Validation Data Gallery

• 

  WB analysis of mouse brain using 11087-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11087-2-AP (IRSp53 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11087-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11087-2-AP (IRSp53 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11087-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11087-2-AP (IRSp53 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11087-2-AP

  IP result of anti-IRSp53 (IP:11087-2-AP, 4ug; Detection:11087-2-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of mouse brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11087-2-AP (IRSp53 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11087-2-AP (IRSp53 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11087-2-AP targets IRSp53 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRSp53 fusion protein Ag1552
• Full Name: BAI1-associated protein 2
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014020
• Gene Symbol: IRSp53
• Gene ID (NCBI): 10458
• RRID: AB_2063075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	WB	Biol Cell; Extracellular vesicles containing the I-BAR protein IRSp53 are released from the cell plasma membrane in an Arp2/3 dependent manner.; Authors - Alice Trausch
hamster	WB,IP	Proteomics; Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell-Cell Transmission.; Authors - Fei-Long Yu
mouse	WB	JCI Insight; Disruption of Robo2-Baiap2 integrated signaling drives cystic disease.; Authors - Qinggang Li
mouse	IF	Lab Invest; FAK Signaling Induces the Onset of Proteinuria via FAK Signaling in a Mouse Model of Lung Cancer; Authors - Sheng-Wen Niu
human	IHC	Elife; Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes; Authors - Aojia Zhuang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (10-28-2019)

Bands detected at the correct molecular weight. Great antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS, A549

IRSp53 Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS, A549 (Cat no:11087-2-AP)

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