Anticorps Monoclonal anti-ATP5A1

• Phare
• Validé par KD/KO

ATP5A1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

22

CloneNo.

1B10H3

Avis (2)

N° de cat : 66037-1-Ig

Synonymes

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Maarker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

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Galerie de données de validation

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  WB analysis using 66037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66037-1-Ig

  WB result of ATP5A1 antibody (66037-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5A1 transfected HepG2 cells.

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  WB analysis of HepG2 using 66037-1-Ig

  Western blot analysis of ATP5A1 in various tissues and cell lines using Proteintech antibody 66037-1-Ig at the dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66037-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66037-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66037-1-Ig (ATP5A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 66037-1-Ig

  IP Result of anti-ATP5A1 (IP:66037-1-Ig, 5ug; Detection:66037-1-Ig 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66037-1-Ig(ATP5A1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66037-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:300 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Red: CL555-phalloidin staining of F-actin. .

• 

  IF Staining of HeLa using 66037-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66037-1-Ig (ATP5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66037-1-Ig

  1X10^6 HeLa cells were stained with 0.2 ug Anti-Human ATP5A1 (66037-1-Ig, Clone:1B10H3) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or stained with 0.2 ug isotype control and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:150-1:600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Informations sur le produit

66037-1-Ig cible ATP5A1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP5A1 Protéine recombinante Ag8119
• Nom complet: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC064562
• Symbole du gène: ATP5A1
• Identification du gène (NCBI): 498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
hamster	IF	Nat Commun; The non-redundant functions of PIWI family proteins in gametogenesis in golden hamsters; Authors - Xiaolong Lv
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
mouse	WB	Diabetes; CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis; Authors - Meng Ding
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (09-12-2022)

Mitochondrial fractions were obtained from whole cell lysates of 2 immortalized human breast cancer cell lines using a commercial kit, leading to pure mitochondrial fractions (ATP5A1 positive) and fractions comprising the cytosols minus the mitochondria (ATP5A1 negative). Primary antibody incubation was performed overnight at 4ºC. Ponceau S is used as total protein loading control.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human breast cancer cells

ATP5A1 Antibody Western Blot validation (1:5000 dilution) in Human breast cancer cells (Cat no:66037-1-Ig)

FH

Cassandr (Verified Customer) (02-10-2022)

Consistently works antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HULEC

ATP5A1 Antibody Immunofluorescence validation (1:250 dilution) in HULEC (Cat no:66037-1-Ig)

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