Anticorps Polyclonal de lapin anti-ATP1A1-Specific

• Phare
• Validé par KD/KO

ATP1A1-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 55187-1-AP

Synonymes

ATP1A1, ATP1A1-Specific, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of HEK-293 using 55187-1-AP

  WB result of ATP1A1-Specific antibody (55187-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1-Specific transfected HEK-293 cells.

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  WB analysis using 55187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55187-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55187-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55187-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55187-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 55187-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 55187-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 55187-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1-Specific antibody (55187-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of Caco-2 using 55187-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 55187-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug ATP1A1-Specific antibody (55187-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules MCF-7, cellules Neuro-2a, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293, cellules Caco-2
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 3 publications below

Informations sur le produit

55187-1-AP cible ATP1A1-Specific dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: NM_000701
• Symbole du gène: ATP1A1
• Identification du gène (NCBI): 476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

Publications

Species	Application	Title
human	IF	Autophagy; Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury; Authors - Yanping Li
mouse	WB	Hypertension; Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.; Authors - Shuai Shao
mouse	IF	Mol Biol Cell; Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.; Authors - Karolina Plössl
human	IF	Sci Rep; Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification.; Authors - Yan Xu
human	WB	PLoS One; A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.; Authors - Miao Liu
human	WB	Exp Eye Res; CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation.; Authors - Yiran Wang

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