ATP1A2 Polyclonal antibody

ATP1A2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 16836-1-AP


alpha 2 ATPase, ATP1A2, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2

Tested Applications

Positive WB detected in37°C incubated mouse heart tissue, 37°C incubated mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, human kidney tissue, human testis tissue, human skin tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.05 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16836-1-AP targets ATP1A2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Haliotis discus hannai, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1A2 fusion protein Ag10515
Full Name ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
Calculated Molecular Weight 1020 aa, 112 kDa
Observed Molecular Weight 97-100 kDa
GenBank Accession NumberBC052271
Gene Symbol ATP1A2
Gene ID (NCBI) 477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2.


Product Specific Protocols
WB protocol for ATP1A2 antibody 16836-1-APDownload protocol
IHC protocol for ATP1A2 antibody 16836-1-APDownload protocol
IF protocol for ATP1A2 antibody 16836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

Authors - Yi-Ing Chen

Eur J Nucl Med Mol Imaging

Tumour blood flow for prediction of human prostate cancer aggressiveness: a study with Rubidium-82 PET, MRI and Na+/K+-ATPase-density.

Authors - Mads Ryø Jochumsen

J Cereb Blood Flow Metab

Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter in electrolyte fluxes in an in vitro blood-brain barrier model of dehydration.

Authors - Kasper Lykke


The α2β2 isoform combination dominates the astrocytic Na(+) /K(+) -ATPase activity and is rendered nonfunctional by the α2.G301R familial hemiplegic migraine type 2-associated mutation.

Authors - Anca Stoica

Biomed Pharmacother

Rebound effects of NCX3 pharmacological inhibition: A novel strategy to accelerate myelin formation in oligodendrocytes.

Authors - Mariarosaria Cammarota

J Cell Mol Med

Comprehensive analysis identified a reduction in ATP1A2 mediated by ARID3A in abdominal aortic aneurysm.

Authors - Qunhui Wang