ATP1B1 Polyclonal antibody

ATP1B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 15192-1-AP

Synonyms

Sodium/potassium-transporting ATPase subunit beta-1, Sodium/potassium-dependent ATPase subunit beta-1, Na+/K+ ATPase beta 1 subunit, Na, K-ATPase β1, Beta 1 subunit of Na(+) K(+) ATPase

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Validation Data Gallery

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  WB analysis of mouse brain using 15192-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15192-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15192-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15192-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15192-1-AP (ATP1B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15192-1-AP

  IP result of anti-ATP1B1 (IP:15192-1-AP, 4ug; Detection:15192-1-AP 1:2000) with mouse brain tissue lysate 1600 ug.

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  IHC staining of human brain using 15192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15192-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 15192-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15192-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15192-1-AP (ATP1B1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15192-1-AP

  Immunofluorescent analysis of HEK-293 cells using 15192-1-AP (ATP1B1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, human brain tissue, mouse heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

15192-1-AP targets ATP1B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP1B1 fusion protein Ag7279
• Full Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC000006
• Gene Symbol: ATP1B1
• Gene ID (NCBI): 481
• RRID: AB_2290040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

Publications

Species	Application	Title
human	IF, WB	J Immunol; Inducible ATP1B1 Upregulates Antiviral Innate Immune Responses by the Ubiquitination of TRAF3 and TRAF6.; Authors - Wei Cao
mouse	IF	Mol Biol Cell; Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.; Authors - Karolina Plössl
human,mouse	WB,IHC	Front Pharmacol; High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease; Authors - Meina Zou
rat	WB	Int J Mol Sci; RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes.; Authors - Akihito Morinaga
mouse	WB	Obesity (Silver Spring); 27-Hydroxycholesterol Inhibits Sterol Regulatory Element-Binding Protein 1 Activation and Hepatic Lipid Accumulation in Mice.; Authors - Di Li
human	WB	Exp Eye Res; Pathomechanism of mutated and secreted retinoschisin in X-linked juvenile retinoschisis.; Authors - Karolina Plössl