ATP1B2 Polyclonal antibody

ATP1B2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 22338-1-AP

Synonyms

AMOG, ATP1B2, ATPase Na+/K+ beta 2

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Validation Data Gallery

• 

  WB analysis of mouse brain using 22338-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 22338-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 22338-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22338-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22338-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 22338-1-AP

  IP result of anti-ATP1B2 (IP:22338-1-AP, 4ug; Detection:22338-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human brain using 22338-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22338-1-AP (ATP1B2 Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 22338-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22338-1-AP (ATP1B2 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, C2C12 cell, C6 cells, mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 2 publications below

Product Information

The immunogen of 22338-1-AP is ATP1B2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP1B2 fusion protein Ag17818
• Full Name: ATPase, Na+/K+ transporting, beta 2 polypeptide
• Calculated Molecular Weight: 290 aa, 33 kDa
• Observed Molecular Weight: 45-65 kDa
• GenBank Accession Number: BC126175
• Gene Symbol: ATP1B2
• Gene ID (NCBI): 482
• RRID: AB_2879077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1B2 is the β2 subunit of Na+/K+-ATPase which is an essential membrane-bound enzyme responsible for the transport of Na+ and K+ in most eukaryotic cells. ATP1B2 is also called the adhesion molecule on glia (AMOG) and it is highly expressed in normal glia. It is a heavily glycosylated protein that plays a role in cellular adhesion in the CNS. Recently differential expression of ATP1B2 has been found in some glioneuronal tumors (PMID: 23887941, 19371356). This antibody recognizes the endogenous ATP1B2 protein in human brain. The bands between 45 kDa and 65 kDa represent the glycosylated forms of ATP1B2 in different levels (PMID: 8918259).

Publications

Species	Application	Title
mouse	IF	Cell Mol Gastroenterol Hepatol; Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.; Authors - Yuli Wang
rat	WB	Redox Biol; Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration.; Authors - Xiaoying Luo
human	WB	Cancer Cell Int; Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na+/K+-ATPase β2: potential molecular entities to treat invasive brain cancer.; Authors - Natália Barreto
human,rat	WB	World J Biol Psychiatry; Altered expression levels of miR-144-3p and ATP1B2 are associated with schizophrenia.; Authors - Bo Pan
human	WB	Eur J Appl Physiol; Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.; Authors - Muath M Altarawneh
rat	WB	Neurosci Lett; Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway; Authors - Bo Pan