Anticorps Polyclonal de lapin anti-ASS1

• Phare
• Validé par KD/KO

ASS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 16210-1-AP

Synonymes

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

---

Galerie de données de validation

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 16210-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16210-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16210-1-AP

  IP result of anti-ASS1 (IP:16210-1-AP, 4ug; Detection:16210-1-AP 1:1500) with HeLa cells lysate 1600ug.

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 16210-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16210-1-AP (ASS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules SMMC-7721, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du foie humain, tissu hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 13 publications below
• IF: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

16210-1-AP cible ASS1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASS1 Protéine recombinante Ag9200
• Nom complet: argininosuccinate synthetase 1
• Masse moléculaire calculée: 412 aa, 47 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC009243
• Symbole du gène: ASS1
• Identification du gène (NCBI): 445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Publications

Species	Application	Title
mouse	IF	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
human	WB	Sci Transl Med; An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.; Authors - Wei-Jian Li
human	WB	Mol Cell; Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants; Authors - Chao Yang
human	WB	Mol Cell; CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.; Authors - Ran Lin ; KO Validated
mouse	IHC	Autophagy; BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver.; Authors - Maya Z Springer
mouse	IF	J Am Soc Nephrol; Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.; Authors - Sierra S Marable

Produits associés

ASL Polyclonal antibody

16645-1-AP

OTC Polyclonal antibody

26470-1-AP

ADSS Polyclonal antibody

16373-1-AP

CPS1 Polyclonal antibody

24513-1-AP

ASNS Polyclonal antibody

14681-1-AP