ASL Polyclonal antibody

ASL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16645-1-AP

Synonyms

argininosuccinate lyase, Arginosuccinase, ASAL, ASL

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Validation Data Gallery

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  WB analysis using 16645-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16645-1-AP (ASL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16645-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16645-1-AP (ASL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16645-1-AP (ASL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16645-1-AP (ASL Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 16645-1-AP is ASL Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASL fusion protein Ag10007
• Full Name: argininosuccinate lyase
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC008195
• Gene Symbol: ASL
• Gene ID (NCBI): 435
• RRID: AB_2878293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Publications

Species	Application	Title
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
human,mouse	WB	Cell Discov; TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential; Authors - Jing He
mouse	WB	Sci Transl Med; Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin; Authors - PamelaSara E Head
human	WB,IHC	J Proteome Res; Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.; Authors - Yin Cao