ASL Polyclonal antibody

ASL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16645-1-AP

Synonyms

argininosuccinate lyase, Arginosuccinase, ASAL, ASL



Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16645-1-AP is ASL Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASL fusion protein Ag10007
Full Name argininosuccinate lyase
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC008195
Gene Symbol ASL
Gene ID (NCBI) 435
RRIDAB_2878293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Protocols

Product Specific Protocols
WB protocol for ASL antibody 16645-1-APDownload protocol
IHC protocol for ASL antibody 16645-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Metab

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Authors - Wenxiang Zhang
human,mouseWB

Cell Discov

TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential

Authors - Jing He
mouseWB

Sci Transl Med

Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin

Authors - PamelaSara E Head
humanWB,IHC

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao