Anticorps Polyclonal de lapin anti-ASGR1

• Phare
• Validé par KD/KO

ASGR1 Polyclonal Antibody for WB, IHC, IF-P, FC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay

Conjugaison

Non conjugué

Publications

17

Avis (3)

N° de cat : 11739-1-AP

Synonymes

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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Galerie de données de validation

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  WB analysis using 11739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11739-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11739-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11739-1-AP

  IP result of anti-ASGR1 (IP:11739-1-AP, 4ug; Detection:11739-1-AP 1:2000) with mouse liver tissue lysate 4000 ug.

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  IHC staining of rat liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11739-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11739-1-AP (ASGR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using ASGR1 antibody (11739-1-AP) labeled with FlexAble CoraLite Plus 555 Kit (KFA502, orange), CoraLite® Plus 488 CD163 antibody (CL488-16646, green), CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005, magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver cancer using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat liver using 11739-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using ASGR1 antibody (11739-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11739-1-AP

  1x10^6 HepG2 cells were surface stained with 0.4 ug ASGR1 Polyclonal antibody (11739-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu hépatique de rat, tissu de cancer du foie humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du foie humain, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11739-1-AP cible ASGR1 dans les applications de WB, IHC, IF-P, FC, IP, CoIP, ELISA, Blocking assay et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASGR1 Protéine recombinante Ag2310
• Nom complet: asialoglycoprotein receptor 1
• Masse moléculaire calculée: 291 aa, 33 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC032130
• Symbole du gène: ASGR1
• Identification du gène (NCBI): 432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Publications

Species	Application	Title
mouse	FC	Nature; Regulated control of gene therapies by drug-induced splicing.; Authors - Alex Mas Monteys
mouse	FC	Nano Lett; A Potent Branched-Tail Lipid Nanoparticle Enables Multiplexed mRNA Delivery and Gene Editing In Vivo.; Authors - Khalid A Hajj
human	IF	Acta Biomater; Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.; Authors - Kun Du
human	WB	Biomaterials; Dual targeted delivery of statins and nucleic acids by chitosan-based nanoparticles for enhanced antiatherosclerotic efficacy.; Authors - Ting Jiang
human	WB	Cancer Res; ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth.; Authors - Wai Kit Ma
human	IHC,IF	Mol Ther; Enhanced Potency of GalNAc-Conjugated Antisense Oligonucleotides in Hepatocellular Cancer Models.; Authors - Youngsoo Kim

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Mouse liver tissue

FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 11739-1-AP (ASGR1 antibody) at dilution of 1:25000 incubated at 4 degree C overnight. ASGR1 bands appeared at about 42 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Mouse liver tissue

FH

James (Verified Customer) (11-18-2022)

Very good antibody! Used it to label hepatocytes in section of paraffin-embedded liver tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

ASGR1 Antibody Immunofluorescence validation (1/1000 dilution) in Liver (Cat no:11739-1-AP)

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