• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ARL8B

ARL8B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 13049-1-AP

Synonymes

ARL10C, ARL8B, Gie1



Applications testées

Résultats positifs en WBtissu cérébral humain fœtal, cellules NIH/3T3, tissu cérébral de souris, tissu cérébral humain
Résultats positifs en IHCtissu cérébral de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules A549,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

13049-1-AP cible ARL8B dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, poulet, singe, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ARL8B Protéine recombinante Ag3711
Nom complet ADP-ribosylation factor-like 8B
Masse moléculaire calculée 186 aa, 22 kDa
Poids moléculaire observé 22 kDa
Numéro d’acquisition GenBankBC013131
Symbole du gène ARL8B
Identification du gène (NCBI) 55207
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Protocole

Product Specific Protocols
WB protocol for ARL8B antibody 13049-1-APDownload protocol
IHC protocol for ARL8B antibody 13049-1-APDownload protocol
IF protocol for ARL8B antibody 13049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Lysosomal positioning coordinates cellular nutrient responses.

Authors - Korolchuk Viktor I VI
  • KD Validated
humanWB

Nat Commun

DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy

Authors - Rahul Kumar
monkeyWB

Sci Adv

The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.

Authors - Meng Lu
  • KD Validated
mouseWB,IF

Sci Adv

Endosomal sorting drives the formation of axonal prion protein endoggresomes.

Authors - Romain Chassefeyre
  • KO Validated
humanWB

Nat Commun

RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.

Authors - Tal Keren-Kaplan
mouseWB

J Cell Biol

The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.

Authors - Natalia Schiefermeier

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Olivia (Verified Customer) (01-08-2020)

WB: Lysates from mouse bone marrow-derived macrophages were subjected to SDS PAGE followed by western blot with 13049-1-AP(Arl8b antibody) at a dilution of 1:500 overnight at 4°C.IF: Mouse bone marrow-derived macrophages were fixed in 4% PFA and permeabilized with 0.5% saponin before incubation with 1:500 Arl8b antibody (in 1% BSA/0.1% saponin/PBS) overnight at 4°C, followed by incubation with anti-rabbit Alexa Fluor 568 for 1h at room temperature.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse bone marrow-derived macrophages
ARL8B Antibody Western Blot,Immunofluorescence, validation (1:500 dilution) in Mouse bone marrow-derived macrophages (Cat no:13049-1-AP)