CD1d Polyclonal antibody

• Featured Product
• KD/KO Validated

CD1d Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17336-1-AP

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

---

Validation Data Gallery

• 

  WB analysis of Jurkat cells using 17336-1-AP

  WB result of CD1D antibody (17336-1-AP, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

• 

  WB analysis of mouse skin using 17336-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17336-1-AP (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: mouse skin tissue, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 17336-1-AP is CD1d Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD1d fusion protein Ag11371
• Full Name: CD1d molecule
• Calculated Molecular Weight: 335 aa, 38 kDa
• Observed Molecular Weight: 38-40 kDa, 50-55 kDa
• GenBank Accession Number: BC027926
• Gene Symbol: CD1D
• Gene ID (NCBI): 912
• RRID: AB_2878387
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-50 kDa.

Publications

Species	Application	Title
pig	WB,IF	Emerg Microbes Infect; CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis; Authors - Xin Chen