• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anticorps Polyclonal de lapin anti-ALK

ALK Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 24184-1-AP

Synonymes

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using ALK Polyclonal antibody (24184-1-AP)

    WB analysis using 24184-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24184-1-AP (ALK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ALK Polyclonal antibody (24184-1-AP)

    IHC staining of human lymphoma using 24184-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ALK Polyclonal antibody (24184-1-AP)

    IHC staining of human lymphoma using 24184-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ALK Polyclonal antibody (24184-1-AP)

    IHC staining of human brain using 24184-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ALK Polyclonal antibody (24184-1-AP)

    IHC staining of human brain using 24184-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 40x lens).

Applications testées

Résultats positifs en WBcellules Jurkat, cellules MCF-7, cellules MG U-87
Résultats positifs en IHCtissu de lymphome humain, tissu cérébral humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 2 publications below

Informations sur le produit

24184-1-AP cible ALK dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ALK Protéine recombinante Ag21493
Nom complet anaplastic lymphoma receptor tyrosine kinase
Masse moléculaire calculée 1620 aa, 176 kDa
Poids moléculaire observé180 kDa
Numéro d’acquisition GenBankNM_004304
Symbole du gène ALK
Identification du gène (NCBI) 238
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).

Protocole

Product Specific Protocols
WB protocol for ALK antibody 24184-1-APDownload protocol
IHC protocol for ALK antibody 24184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas.

Authors - Mai-Qing Yang
View Article
humanWB

Antiviral Res

Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3

Authors - Tan Ming
View Article