EML4 Polyclonal antibody

EML4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22709-1-AP

Synonyms

Restrictedly overexpressed proliferation-associated protein, EMAPL4, Echinoderm microtubule-associated protein-like 4, C2orf2

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Validation Data Gallery

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  WB analysis of A549 using 22709-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22709-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22709-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 22709-1-AP

  IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.

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  IHC staining of human breast cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22709-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 22709-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EML4 antibody (22709-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of A549 using 22709-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HeLa cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22709-1-AP targets EML4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EML4 fusion protein Ag18463
• Full Name: echinoderm microtubule associated protein like 4
• Calculated Molecular Weight: 981 aa, 109 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC140845
• Gene Symbol: EML4
• Gene ID (NCBI): 27436
• RRID: AB_2879153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.