Anticorps Polyclonal de lapin anti-AFP

AFP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

102

N° de cat : 14550-1-AP

Synonymes

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

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Galerie de données de validation

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  WB analysis using 14550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14550-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14550-1-AP (AFP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14550-1-AP

  IP result of anti-AFP (IP:14550-1-AP, 5ug; Detection:14550-1-AP 1:1000) with HepG2 cells lysate 6000ug.

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14550-1-AP (AFP Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14550-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14550-1-AP (AFP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 14550-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14550-1-AP (AFP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HuH-7, cellules L02, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 28 publications below
• IHC: See 42 publications below
• IF: See 35 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14550-1-AP cible AFP dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AFP Protéine recombinante Ag6089
• Nom complet: alpha-fetoprotein
• Masse moléculaire calculée: 69 kDa
• Poids moléculaire observé: 68-72 kDa
• Numéro d’acquisition GenBank: BC027881
• Symbole du gène: AFP
• Identification du gène (NCBI): 174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

Publications

Species	Application	Title
mouse	IHC	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	IHC	Cancer Cell; Genomic and Transcriptomic Profiling of Combined Hepatocellular and Intrahepatic Cholangiocarcinoma Reveals Distinct Molecular Subtypes.; Authors - Ruidong Xue
mouse	IHC	J Clin Invest; Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.; Authors - Xueru Mu
human	IHC	J Clin Invest; HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis.; Authors - Celine Hernandez
human,mouse	WB,IHC,IF	Nat Commun; Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma.; Authors - Wei Cheng
human	IHC	Nat Commun; Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.; Authors - Jun Lu

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