Anticorps Polyclonal de lapin anti-ADRP/Perilipin 2

• Phare
• Validé par KD/KO

ADRP/Perilipin 2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

122

Avis (6)

N° de cat : 15294-1-AP

Synonymes

ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, PLIN2

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Galerie de données de validation

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  WB analysis using 15294-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15294-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 15294-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin-2 antibody (15294-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 15294-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP/Perilipin 2 antibody (15294-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules K-562, cellules NIH/3T3, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de carcinome à cellules rénales humain, tissu hépatique de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa traitées à l'acide oléique,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:200-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 76 publications below
• IHC: See 21 publications below
• IF: See 47 publications below

Informations sur le produit

15294-1-AP cible ADRP/Perilipin 2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, poisson-zèbre, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADRP/Perilipin 2 Protéine recombinante Ag7539
• Nom complet: adipose differentiation-related protein
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC005127
• Symbole du gène: Perilipin-2
• Identification du gène (NCBI): 123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Publications

Species	Application	Title
human	IF	Nature; iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer; Authors - Dong Shin Park
human	IF	Nature; The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.; Authors - Simona Ricciardi
mouse,human	IF,IHC	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	IF	Nat Commun; Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy; Authors - Donghai Cui
mouse	WB	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He
mouse	WB	Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Liliana (Verified Customer) (02-13-2024)

WORKS VERY WELL ON PH9, 1:2000 AND ON PH6, 1:1000

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: SKIN WITH SEBACCEUS ADENOMA

ADRP/Perilipin 2 Antibody Immunohistochemistry validation (1:2000 dilution) in SKIN WITH SEBACCEUS ADENOMA (Cat no:15294-1-AP)

FH

Christine (Verified Customer) (09-19-2023)

Tried on HEK293T cells treated with 300 uM oleic acid overnight to induce the formation of lipid droplets. This antibody decorates some droplets but was a bit weak. TIP47 antibody (10694-1-AP) performed better (clearer detection of the droplets) at the same concentration (1:100).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293T

FH

Kamal (Verified Customer) (07-05-2023)

Worked well when diluted with 1X PBS.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Liver tissue

FH

Christin (Verified Customer) (03-09-2023)

Great antibody for WB with low background signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human iPSC-derived microglia and macrophages

FH

Nick (Verified Customer) (04-20-2022)

Helped localize expression of Plin2

• Applications: Western Blot, Immunoprecipitation, Cell culture
• Cell Tissue Type: 3T3-L1

FH

Boyan (Verified Customer) (05-09-2019)

Very good for WB

• Applications: Western Blot,
• Cell Tissue Type: MEF

Produits associés

TIP47 Polyclonal antibody

10694-1-AP

ADRP/Perilipin-2 Monoclonal antibody

60340-1-Ig

Perilipin 5 Polyclonal antibody

26951-1-AP