Perilipin 5 Polyclonal antibody

• Featured Product
• KD/KO Validated

Perilipin 5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 26951-1-AP

Synonyms

LSDP5, OXPAT, Perilipin 5, PLIN5

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Validation Data Gallery

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  WB analysis using 26951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26951-1-AP

  WB result of Perilipin 5 antibody (26951-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.

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  WB analysis of mouse liver using 26951-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 26951-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: MCF-7 cells, pig heart tissue, mouse liver tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

26951-1-AP targets Perilipin 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Perilipin 5 fusion protein Ag25272
• Full Name: lipid storage droplet protein 5
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC131524
• Gene Symbol: Perilipin 5
• Gene ID (NCBI): 440503
• RRID: AB_2880699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.

Publications

Species	Application	Title
mouse	WB	J Mol Cell Biol; Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice; Authors - Xinyu Bao
mouse	WB	EMBO Rep; miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle.; Authors - Hui Wang
mouse	WB	Int J Mol Sci; Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.; Authors - Miljenko V Panajatovic
mouse	WB	Int Immunopharmacol; Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway.; Authors - Kang Huo
human	WB,IF	Hum Cell; Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.; Authors - Hsueh-Chun Wang
mouse	WB	Biochem Biophys Res Commun; Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.; Authors - Jie Feng