Perilipin 5 Polyclonal antibody

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• Perilipin 5 Polyclonal antibody (26951-1-AP)

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• KD/KO Validated

Perilipin 5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26951-1-AP

Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

LSDP5, OXPAT, PAT-1, Perilipin-5, PLIN5

• 

  WB analysis using 26951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 26951-1-AP

  WB result of Perilipin 5 antibody (26951-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 26951-1-AP

  WB result of Perilipin 5 antibody (26951-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Perilipin 5 transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of pig heart using 26951-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 26951-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26951-1-AP (Perilipin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of MCF-7 using 26951-1-AP

  IP result of anti-Perilipin 5 (IP:26951-1-AP, 4ug; Detection:26951-1-AP 1:8000) with MCF-7 cells lysate 1200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brown adipose using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26951-1-AP (Perilipin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 26951-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26951-1-AP (Perilipin 5 antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse liver tissue, pig heart tissue, mouse heart tissue, rat heart tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26951-1-AP targets Perilipin 5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25272

  Product name: Recombinant human LSDP5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 381-463 aa of BC131524

  Sequence: ILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF

• Full Name: lipid storage droplet protein 5
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC131524
• Gene Symbol: Perilipin 5
• Gene ID (NCBI): 440503
• RRID: AB_2880699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q00G26
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cancer Cell; Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma.; Authors - Flavia Bernardi
mouse	WB	Nat Metab; Daytime-restricted feeding enhances running endurance without prior exercise in mice; Authors - Haoran Xin
		Nat Commun; O-GlcNAc transferase plays dual antiviral roles by integrating innate immunity and lipid metabolism; Authors - Hong Dong
mouse	WB	Metabolism; Mitofusin-2 induced by exercise modifies lipid droplet-mitochondria communication, promoting fatty acid oxidation in male mice with NAFLD; Authors - Juan Carlos Bórquez
mouse,human	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du
mouse	WB,IF,CoIP	Theranostics; Chronic intermittent hypoxia increases Parkinson's disease susceptibility via PPARα-mediated lipid droplet-mitochondrial dysfunction.; Authors - Ming-Rui Zhai