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TIP47 Polyclonal antibody

TIP47 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10694-1-AP

Synonyms

47 kDa MPR binding protein, Cargo selection protein TIP47, M6PRBP1, Perilipin 3, Placental protein 17, PLIN3, PP17, TIP47



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, 3T3-L1 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMEF cells, oleic acid treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10694-1-AP targets TIP47 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIP47 fusion protein Ag1028
Full Name mannose-6-phosphate receptor binding protein 1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC007566
Gene Symbol TIP47/PLIN3
Gene ID (NCBI) 10226
RRIDAB_2297252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
WB protocol for TIP47 antibody 10694-1-APDownload protocol
IHC protocol for TIP47 antibody 10694-1-APDownload protocol
IF protocol for TIP47 antibody 10694-1-APDownload protocol
IP protocol for TIP47 antibody 10694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo
mouseWB

Nat Commun

Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.

Authors - Fangjun Yu
humanIF

Nat Commun

The autophagy protein ATG9A enables lipid mobilization from lipid droplets.

Authors - Elodie Mailler
humanWB

Hepatology

CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis.

Authors - Cédric Langhi
mouseWB

Nat Commun

O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.

Authors - Yunfan Yang
humanIF

J Cell Biol

A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover.

Authors - Eastman Scott W SW

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (09-19-2023)

Tested on HEK293T cells stimulated by 300 uM oleic acid overnight to stimulate the formation of lipid droplets. This antibody (tested only at 1:100 so far) decorates the lipid droplets, it worked well.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HEK293T