TIP47 Polyclonal antibody

• Featured Product
• KD/KO Validated

TIP47 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 10694-1-AP

Synonyms

M6PRBP1, Perilipin 3, Perilipin-3, Mannose-6-phosphate receptor-binding protein 1, 47 kDa MPR-binding protein

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Validation Data Gallery

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  WB analysis using 10694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10694-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10694-1-AP

  IP result of anti-TIP47 (IP:10694-1-AP, 3ug; Detection:10694-1-AP 1:1000) with HeLa cells lysate 1000ug.

• 

  IF Staining of MEF cells using 10694-1-AP

  IF result of anti-TIP47 (10694-1-AP,1:500) with MEF cell by Dr.Hector Alex Saka.

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  IF Staining of HeLa using 10694-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (10694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10694-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TIP47 antibody (10694-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: MEF cells, oleic acid treated HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Product Information

10694-1-AP targets TIP47 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIP47 fusion protein Ag1028
• Full Name: mannose-6-phosphate receptor binding protein 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC007566
• Gene Symbol: TIP47/PLIN3
• Gene ID (NCBI): 10226
• RRID: AB_2297252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Publications

Species	Application	Title
human	IF	Nat Commun; The autophagy protein ATG9A enables lipid mobilization from lipid droplets.; Authors - Elodie Mailler
human	WB	Hepatology; CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis.; Authors - Cédric Langhi
human	IF	J Cell Biol; A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover.; Authors - Eastman Scott W SW
	WB	J Hepatol; Perilipin-5 is regulated by statins and controls triglyceride contents in the hepatocyte.; Authors - Cédric Langhi
human	WB	Cancer Res; KRAS Controls Pancreatic Cancer Cell Lipid Metabolism and Invasive Potential through the Lipase HSL.; Authors - Cody N Rozeveld
human	WB	EMBO J; COPI-TRAPPII activates Rab18 and regulates its lipid droplet association.; Authors - Chunman Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (09-19-2023)

Tested on HEK293T cells stimulated by 300 uM oleic acid overnight to stimulate the formation of lipid droplets. This antibody (tested only at 1:100 so far) decorates the lipid droplets, it worked well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293T

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