Endostatin Polyklonaler Antikörper

Endostatin Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 18301-1-AP

Synonyme

COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, Endostatin, KNO, KNO1

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Galerie der Validierungsdaten

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  WB analysis of human liver using 18301-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 18301-1-AP (Endostatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 18301-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18301-1-AP (Endostatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 18301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18301-1-AP (Endostatin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 18301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18301-1-AP (Endostatin antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

18301-1-AP bindet in WB, IHC, IF, ELISA Endostatin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type XVIII, alpha 1
• Berechnetes Molekulargewicht: 178 kDa
• Beobachtetes Molekulargewicht: 20-70 kDa
• GenBank-Zugangsnummer: BC063833
• Gene symbol: COL18A1
• Gene ID (NCBI): 80781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain (COL18A1). It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It potently inhibits endothelial cell proliferation and inhibits endothelial cell angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Endostatin is a broad spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). This antibody raised against 1693-1706 aa of human collagen alpha-1(XVIII) chain can recognize endostatin (20 kDa) and several endostatin-related fragments of higher molecular weight (PMID: 15545970; 19358707).

Publikationen

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Cancer Lett; Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying IL-24/endostatin enhances glioma therapy.; Authors - Junhe Zhang
human	WB	FASEB J; MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.; Authors - Pei Zhang
human	WB	Biotechnol Lett; PMID: 26830094; Authors - Baolong Wang
rat	WB	Neural Regen Res; miR-181b promotes angiogenesis and neurological function recovery after ischemic stroke; Authors - Li-Xia Xue

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