ZCCHC11 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZCCHC11 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 18980-1-AP

Synonyme

KIAA0191, PAPD3, Terminal uridylyltransferase 4, TUT4, TUTase 4, ZCCHC11

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 18980-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18980-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18980-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 18980-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18980-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18980-1-AP (ZCCHC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 18980-1-AP

  IP Result of anti-ZCCHC11 (IP:18980-1-AP, 5ug; Detection:18980-1-AP 1:1000) with MCF-7 cells lysate 2000ug.

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  IF Staining of HepG2 using 18980-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18980-1-AP (ZCCHC11 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 23 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

18980-1-AP bindet in WB, IP, IF, CoIP, ELISA ZCCHC11 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: zinc finger, CCHC domain containing 11
• Berechnetes Molekulargewicht: 185 kDa
• Beobachtetes Molekulargewicht: 185 kDa
• GenBank-Zugangsnummer: NM_015269
• Gene symbol: ZCCHC11
• Gene ID (NCBI): 23318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.

Publikationen

Species	Application	Title
mouse	WB	Nature; mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome.; Authors - Marcos Morgan ; KO Validated
mouse	WB	Nature; A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway.; Authors - Chang Hao-Ming HM ; KD Validated
human	WB	Cell; Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.; Authors - Piskounova Elena E ; KD Validated
human	WB,IF	Cell; Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.; Authors - Zbigniew Warkocki
human	WB	Mol Cell; 3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.; Authors - Acong Yang ; KO Validated
human	WB	Mol Cell; A Mechanism for microRNA Arm Switching Regulated by Uridylation.; Authors - Haedong Kim ; KD Validated

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