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LIN28 Polyclonal antibody

LIN28 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11724-1-AP

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1



Tested Applications

Positive WB detected inK-562 cells, NCCIT cells, mouse embryo tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman embronic stem cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11724-1-AP targets LIN28 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN28 fusion protein Ag2312
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 209 aa, 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC028566
Gene symbol LIN28
Gene ID (NCBI) 79727
RRIDAB_2135039
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Protocols

Product Specific Protocols
WB protocol for LIN28 antibody 11724-1-APDownload protocol
IHC protocol for LIN28 antibody 11724-1-APDownload protocol
IP protocol for LIN28 antibody 11724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

A role for Lin28 in primordial germ-cell development and germ-cell malignancy.

Authors - West Jason A JA
human,mouseWB,IHC

Nat Genet

Lin28 promotes transformation and is associated with advanced human malignancies.

Authors - Viswanathan Srinivas R SR
mouseIHC

Nat Genet

Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies.

Authors - Zhu Hao H
humanWB,IF

Nat Methods

A nonviral minicircle vector for deriving human iPS cells.

Authors - Jia Fangjun F
humanIF

Cell Stem Cell

Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.

Authors - Carmen Lorenz
mouseIHC

Cell Res

Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis.

Authors - Phillip J Hsu