YME1L1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YME1L1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

72

Rezensionen (1)

Kat-Nr. : 11510-1-AP

Synonyme

Meg 4, FTSH1, FTSH, EC:3.4.24.-, ATP-dependent zinc metalloprotease YME1L1

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Galerie der Validierungsdaten

• 

  WB analysis using 11510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11510-1-AP

  WB result of YME1L1 antibody (11510-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells.

• 

  IP experiment of HeLa using 11510-1-AP

  IP result of anti-YME1L1 (IP:11510-1-AP, 4ug; Detection:11510-1-AP 1:500) with HeLa cells lysate 1280 ug.

• 

  IF Staining of HepG2 using 11510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YME1L1 antibody (11510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 14 publications below
• WB: See 62 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11510-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA YME1L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YME1L1 fusion protein Ag2069
• Vollständiger Name: YME1-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 716 aa, 80 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC023507
• Gene symbol: YME1L1
• Gene ID (NCBI): 10730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.; Authors - Tao Wang ; KD Validated
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	IF	Mol Cell; Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress.; Authors - Raul Jobava

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christopher (Verified Customer) (03-08-2022)

incubated for 1 hour at RT. produced a weak signal at the expected molecular weight that was sensitive to a YME1L1 targeting sgRNA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

YME1L1 Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11510-1-AP)

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