VAPB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VAPB Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Rezensionen (1)

Kat-Nr. : 14477-1-AP

Synonyme

ALS8, VAMP associated protein B/C, VAMP B, VAMP B/VAMP C, VAMP C, VAP B, VAP B/VAP C, VAP C, VAPB

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 14477-1-AP

  WB result of VAPB antibody (14477-1-AP, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

• 

  WB analysis using 14477-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14477-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14477-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14477-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14477-1-AP

  IP Result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14477-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAPB antibody (14477-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 14477-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VAPB antibody (14477-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 14477-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug VAPB antibody (14477-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslebergewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 1 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

14477-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA VAPB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VAPB fusion protein Ag5857
• Vollständiger Name: VAMP (vesicle-associated membrane protein)-associated protein B and C
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC001712
• Gene symbol: VAPB
• Gene ID (NCBI): 9217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.

Publikationen

Species	Application	Title
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
monkey	WB	Mol Cell; MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.; Authors - Christophe A C Freyre
human	WB	J Clin Invest; SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.; Authors - Museer A Lone
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (09-22-2020)

The antibody have predicted band on western blot.

• Applications: Western Blot
• Cell Tissue Type: Mouse heart

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