Tom22 Polyklonaler Antikörper

Tom22 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11278-1-AP

Synonyme

1C9 2, hTom22, MST065, MSTP065, TOM22, TOMM22

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11278-1-AP

  HeLa and K-562 cells were subjected to SDS PAGE followed by western blot with 11278-1-AP (Tom22 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11278-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11278-1-AP (Tom22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11278-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11278-1-AP (Tom22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11278-1-AP (Tom22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11278-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11278-1-AP (Tom22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11278-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11278-1-AP (Tom22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa, K-562 cells, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 3 publications below

Produktinformation

11278-1-AP bindet in WB, IHC, IF, ELISA Tom22 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Tom22 fusion protein Ag1797
• Vollständiger Name: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15-22 kDa
• GenBank-Zugangsnummer: BC009363
• Gene symbol: TOMM22
• Gene ID (NCBI): 56993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

Publikationen

Species	Application	Title
human	WB	Cell Res; Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.; Authors - Bo Zhou
human	WB,IF	Cell Discov; Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis.; Authors - Fan Yu
human	WB	J Genet Genomics; HPDL deficiency causes a neuromuscular disease by impairing the mitochondrial respiration.; Authors - Yu Sun
human	WB	Mol Biol Cell; Mitochondrial E3 ubiquitin ligase MARCH5 controls mitochondrial fission and cell sensitivity to stress-induced apoptosis through regulation of MiD49 protein.; Authors - Shan Xu
human	WB	J Hum Genet; Mutations in TOMM70 lead to multi-OXPHOS deficiencies and cause severe anemia, lactic acidosis, and developmental delay.; Authors - Xiujuan Wei
human	WB	EMBO Rep; UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy; Authors - Jing Zheng

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