Syntaxin 11 Polyklonaler Antikörper

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• KD/KO Validated

Syntaxin 11 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 13301-1-AP

Synonyme

Syntaxin-11, STX11

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Galerie der Validierungsdaten

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  WB analysis using 13301-1-AP

  Jurkat cells lysates were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13301-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 13301-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 13301-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13301-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13301-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13301-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 13301-1-AP

  IP result of anti-Syntaxin 11 (IP:13301-1-AP, 4ug; Detection:13301-1-AP 1:1000) with DU 145 cells lysate 1280 ug.

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  IHC staining of human lymphoma using 13301-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13301-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13301-1-AP (Syntaxin 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, DU 145-Zellen, humanes Plazenta-Gewebe, humanes Skelettmuskelgewebe, L02-Zellen, Mauslungengewebe
• Erfolgreiche IP: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

13301-1-AP bindet in WB, IP, IF, IHC, ELISA Syntaxin 11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntaxin 11 fusion protein Ag4097
• Vollständiger Name: syntaxin 11
• Berechnetes Molekulargewicht: 287 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33-35 kDa
• GenBank-Zugangsnummer: BC033519
• Gene symbol: Syntaxin 11
• Gene ID (NCBI): 8676
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286).

Publikationen

Species	Application	Title
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
rat	WB,IF	Proc Natl Acad Sci U S A; Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.; Authors - Bin Na-Ryum NR
human	WB,IF, IP	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB, IHC	J Cell Sci; Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis.; Authors - Na-Ryum Bin
human	WB	Front Immunol; Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.; Authors - Jamie A Lopez

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