SREBF2 Polyklonaler Antikörper

SREBF2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 14508-1-AP

Synonyme

bHLHd2, SREBF2, SREBP 2, SREBP2

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 14508-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14508-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14508-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14508-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 14508-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14508-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14508-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14508-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14508-1-AP

  IP Result of anti-SREBF2 (IP:14508-1-AP, 3ug; Detection:14508-1-AP 1:500) with HeLa cells lysate 3000ug.

• 

  IHC staining of mouse brain using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14508-1-AP (SREBF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14508-1-AP (SREBF2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Nierengewebe, Mausnierengewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 31 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

14508-1-AP bindet in WB, IP, IHC, chIP, ELISA SREBF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SREBF2 fusion protein Ag5967
• Vollständiger Name: sterol regulatory element binding transcription factor 2
• Berechnetes Molekulargewicht: 124 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC056158
• Gene symbol: SREBF2
• Gene ID (NCBI): 6721
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.

Publikationen

Species	Application	Title
mouse	WB	Nature; The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.; Authors - Jinbo Han
mouse	WB	Nature; TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.; Authors - Joanne Hsieh
mouse	WB	Environ Pollut; Prenatal exposure to diesel exhaust PM2.5 programmed non-alcoholic fatty liver disease differently in adult male offspring of mice fed normal chow and a high-fat diet.; Authors - Xiaoke Wang
human, mouse	WB	Arterioscler Thromb Vasc Biol; RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.; Authors - Yan-Wei Hu
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
mouse	IHC	J Lipid Res; Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein.; Authors - Ya-Qi Wang

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