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CD43 Polyklonaler Antikörper

CD43 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13959-1-AP

Synonyme

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of K-562 cells using CD43 Polyclonal antibody (13959-1-AP)

    WB analysis of K-562 using 13959-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13959-1-AP (CD43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CD43 Polyclonal antibody (13959-1-AP)

    WB analysis of Jurkat using 13959-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13959-1-AP (CD43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD43 Polyclonal antibody (13959-1-AP)

    IHC staining of human lymphoma using 13959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13959-1-AP (CD43 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD43 Polyclonal antibody (13959-1-AP)

    IHC staining of human lymphoma using 13959-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13959-1-AP (CD43 antibody) at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBK-562-Zellen, Jurkat-Zellen
Erfolgreiche Detektion in IHChumanes Lymphomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13959-1-AP bindet in WB, IHC, ELISA CD43 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD43 fusion protein Ag4836
Vollständiger Name sialophorin
Berechnetes Molekulargewicht 400 aa, 43 kDa
Beobachtetes Molekulargewicht 60-66 kDa
GenBank-ZugangsnummerBC012350
Gene symbol CD43
Gene ID (NCBI) 6693
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

Protokolle

Produktspezifische Protokolle
WB protocol for CD43 antibody 13959-1-APProtokoll herunterladen
IHC protocol for CD43 antibody 13959-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen