SOCS3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SOCS3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (4)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

64

Kat-Nr. : 14025-1-AP

Synonyme

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14025-1-AP

  WB result of SOCS3 antibody (14025-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOCS3 transfected HeLa cells.

• 

  WB analysis using 14025-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14025-1-AP (SOCS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14025-1-AP

  IP result of anti-SOCS3 (IP:14025-1-AP, 4ug; Detection:14025-1-AP 1:500) with K-562 cells lysate 1720ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 56 publications below
• IHC: See 11 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14025-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA SOCS3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SOCS3 fusion protein Ag5170
• Vollständiger Name: suppressor of cytokine signaling 3
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC060858
• Gene symbol: SOCS3
• Gene ID (NCBI): 9021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The suppressor of cytokine signaling (SOCS) family proteins are negative feedback system that inhibits cytokine signal transduction. One of the SOCS family, SOCS3, also named CIS3 and SSI3, is involved in inhibition of JAK/STAT pathway which regulates IL-6 signaling in vivo. SOCS3 can be rapidly trigger by a number of factors including interleukins, IFN-gamma and TNF-alpha. Pathology such as inflammatory response, wound repair and obesity and so on have been linked to SOCS3. Observed MW of SOCS3 is from 22-30 kDa(PMID: 24827536,PMID: 26260587, PMID: 26797799), consistent with the result of Catalog#14025-1-AP.

Publikationen

Species	Application	Title
human	WB	Redox Biol; TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes; Authors - Marwa Ben-Khemis
mouse	WB	Cell Death Differ; TLR2-dependent selective autophagy regulates NF-κB lysosomal degradation in hepatoma-derived M2 macrophage differentiation.; Authors - Chang C-P CP
mouse	WB,IHC	Kidney Int; Ablation of proximal tubular suppressor of cytokine signaling 3 enhances tubular cell cycling and modifies macrophage phenotype during acute kidney injury.; Authors - Nathan Susnik
mouse	WB	Oncoimmunology; Transcriptional factor EB regulates macrophage polarization in the tumor microenvironment.; Authors - Liang Fang
human	WB	PLoS Pathog; Negative regulation of type I interferon signaling by integrin-linked kinase permits dengue virus replication; Authors - Yi-Sheng Kao
mouse	WB	PLoS Genet; PMID: 27711113; Authors - Xiaolin Lin

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