P-selectin / CD62P Monoklonaler Antikörper

P-selectin / CD62P Monoklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

CloneNo.

3G2C7

Kat-Nr. : 60322-1-Ig

Synonyme

CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selectin, SELP

---

Galerie der Validierungsdaten

• 

  WB analysis using 60322-1-Ig

  Untreated and PNGase F-treated lysates of human platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of human peripheral blood platelets using 60322-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60322-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60322-1-Ig (P-selectin / CD62P antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 60322-1-Ig

  IP Result of anti-P-selectin / CD62P (IP:60322-1-Ig, 5ug; Detection:60322-1-Ig 1:600) with K-562 cells lysate 1760ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets, Jurkat-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• FC: See 4 publications below

Produktinformation

60322-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA P-selectin / CD62P und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: P-selectin / CD62P fusion protein Ag4124
• Vollständiger Name: selectin P (granule membrane protein 140kDa, antigen CD62)
• Berechnetes Molekulargewicht: 830 aa, 91 kDa
• Beobachtetes Molekulargewicht: 140 kDa, 105-110 kDa
• GenBank-Zugangsnummer: BC028067
• Gene symbol: SELP
• Gene ID (NCBI): 6403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Publikationen

Species	Application	Title
rat	WB	Acta Biomater; Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection.; Authors - Wenyan Yu
human	WB	Front Oncol; An Essential NRP1-Mediated Role for Tagln2 in Gastric Cancer Angiogenesis.; Authors - Hongwei Jin
mouse	WB	Front Oncol; Platelet Membranes Coated Gold Nanocages for Tumor Targeted Drug Delivery and Amplificated Low-Dose Radiotherapy.; Authors - Mingzhu Chen
human	FC	Commun Biol; Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.; Authors - LinXi Cheng
mouse	WB	Front Bioeng Biotechnol; Nano-Platelets as an Oxygen Regulator for Augmenting Starvation Therapy Against Hypoxic Tumor.; Authors - Chunyu Huang
human	WB	Biol Res; CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis.; Authors - Xiaohao Zhang

Verwandte Produkte

P-selectin / CD62P Polyclonal antibody

13304-1-AP

SELPLG Polyclonal antibody

23605-1-AP

E-selectin / CD62E Polyclonal antibody

20894-1-AP

VCAM-1 Monoclonal antibody

66294-1-Ig

VCAM-1 Polyclonal antibody

11444-1-AP

ICAM-1 Monoclonal antibody

60299-1-Ig

SNX17 Polyclonal antibody

10275-1-AP