SNX17 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNX17 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10275-1-AP

Synonyms

Sorting nexin-17, KIAA0064

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Validation Data Gallery

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  WB analysis using 10275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10275-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10275-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10275-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10275-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10275-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10275-1-AP (SNX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10275-1-AP

  IP result of anti-SNX17 (IP:10275-1-AP, 4ug; Detection:10275-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10275-1-AP (SNX17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse cerebellum tissue, human brain tissue, mouse skin tissue, mouse brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 18 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Product Information

10275-1-AP targets SNX17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNX17 fusion protein Ag0355
• Full Name: sorting nexin 17
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002524
• Gene Symbol: SNX17
• Gene ID (NCBI): 9784
• RRID: AB_2192394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide conversion mechanism for exit from endosomes.; Authors - Katharina Ketel ; KD Validated
mouse	WB	Nat Cell Biol; Sorting nexin 17 prevents lysosomal degradation of β1 integrins by binding to the β1-integrin tail.; Authors - B?ttcher Ralph Thomas RT ; KD Validated
human	WB	Nat Cell Biol; Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.; Authors - Kerrie E McNally
human	WB	Nat Cell Biol; Recycling of autophagosomal components from autolysosomes by the recycler complex.; Authors - Chuchu Zhou
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	WB	Proc Natl Acad Sci U S A; LRP1 is a receptor for Clostridium perfringens TpeL toxin indicating a two-receptor model of clostridial glycosylating toxins.; Authors - Björn Schorch ; KD Validated

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