SECISBP2 Polyklonaler Antikörper

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• KD/KO Validated

SECISBP2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12798-1-AP

Synonyme

Selenocysteine insertion sequence-binding protein 2, SECIS-binding protein 2, SECIS binding protein 2, SBP2

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 12798-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12798-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12798-1-AP (SECISBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12798-1-AP

  IP result of anti-SECISBP2 (IP:12798-1-AP, 3ug; Detection:12798-1-AP 1:600) with mouse testis tissue lysate 8000ug.

• 

  IF Staining of HeLa using 12798-1-AP

  Immunofluorescent analysis of HeLa cells using 12798-1-AP (SECISBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Nierengewebe, Jurkat-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

12798-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SECISBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SECISBP2 fusion protein Ag3541
• Vollständiger Name: SECIS binding protein 2
• Berechnetes Molekulargewicht: 854 aa, 95 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC036109
• Gene symbol: SECISBP2
• Gene ID (NCBI): 79048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Selenium (Se) is an essential trace element required for the biosynthesis of selenoproteins, and selenocysteine insertion sequence (SECIS) binding protein 2 (SECISBP2, or SBP2) represents a key trans-acting factor for the cotranslational insertion of selenocysteine into selenoproteins. Defects in SBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) associated with a reduction in type II iodothyronine deiodinase activity. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Cells depleted of SBP2 have increased levels of ROS, which lead to cellular oxidative stress manifested as DNA lesions, stress granules, and lipid peroxidation, induction of caspase- and cytochrome c-dependent apoptosis, indicating that SBP2 is required for protection against ROS-induced cellular damage and cell survival.

Publikationen

Species	Application	Title
human	WB	Science; SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding.; Authors - Hsiu-Chuan Lin
human	WB	Nat Chem Biol; Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.; Authors - Zhipeng Li
human	WB	Nucleic Acids Res; Identification of a novel endogenous long non-coding RNA that inhibits selenoprotein P translation.; Authors - Yuichiro Mita
human	WB,IF	Free Radic Biol Med; Selective up-regulation of human selenoproteins in response to oxidative stress.; Authors - Touat-Hamici Zahia
mouse	WB	Endocrinology; Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.; Authors - Jiao Fu ; KO Validated
human	WB,IF	J Biol Chem; Interplay between Selenium Levels, Selenoprotein Expression and Replicative Senescence in WI-38 Human Fibroblasts.; Authors - Yona Legrain

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