SEC13 Polyklonaler Antikörper

SEC13 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 15397-1-AP

Synonyme

D3S1231E, Protein SEC13 homolog, SEC13, SEC13 homolog (S. cerevisiae), SEC13 like protein 1, SEC13 related protein, SEC13L1, SEC13R

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Galerie der Validierungsdaten

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  WB analysis using 15397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 15397-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15397-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15397-1-AP

  IP Result of anti-SEC13 (IP:15397-1-AP, 3ug; Detection:15397-1-AP 1:1000) with HepG2 cells lysate 3200ug.

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15397-1-AP

  Immunofluorescent analysis of HepG2 cells using 15397-1-AP (SEC13 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15397-1-AP

  Immunofluorescent analysis of HepG2 cells, using 15397-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IF: See 1 publications below

Produktinformation

15397-1-AP bindet in WB, IP, IHC, IF, ELISA SEC13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC13 fusion protein Ag7610
• Vollständiger Name: SEC13 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC002634
• Gene symbol: SEC13
• Gene ID (NCBI): 6396
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

Publikationen

Species	Application	Title
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Nat Commun; Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation; Authors - Xing Chen
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
rat	WB	Proc Natl Acad Sci U S A; TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments.; Authors - Michael G Hanna
human	WB	Proc Natl Acad Sci U S A; Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.; Authors - Liling Niu
human	WB	Cell Rep; The Sar1 GTPase is dispensable for COPII-dependent cargo export from the ER; Authors - William Kasberg

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Subir (Verified Customer) (09-27-2019)

clean and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

SEC13 Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:15397-1-AP)

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