SESN1 Polyclonal antibody

SESN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21668-1-AP

Synonyms

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HEK-293 cells, mouse liver tissue, human testis tissue, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21668-1-AP is SESN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SESN1 fusion protein Ag16462
Full Name sestrin 1
Calculated Molecular Weight 551 aa, 64 kDa
Observed Molecular Weight 66-68 kDa
GenBank Accession NumberBC112036
Gene Symbol SESN1
Gene ID (NCBI) 27244
RRIDAB_10793724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).

Protocols

Product Specific Protocols
WB protocol for SESN1 antibody 21668-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
mouseWB

Science

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Authors - Andrew L Cangelosi

Diabetes

PMID: 25377878

Authors - Rongya Tao
mouseWB

Biochim Biophys Acta Mol Basis Dis

Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner

Authors - Sujuan Liu
humanWB,IHC

Osteoarthritis Cartilage

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.

Authors - T Shen
humanWB

BMC Cancer

Inhibition of mTORC1 through ATF4-induced REDD1 and Sestrin2 expression by Metformin.

Authors - Se-Kyeong Jang