SESN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SESN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 21668-1-AP

Synonyms

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1

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Validation Data Gallery

• 

  WB analysis using 21668-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21668-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21668-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21668-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21668-1-AP (SESN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, HEK-293 cells, mouse liver tissue, human testis tissue, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 2 publications below

Product Information

21668-1-AP targets SESN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SESN1 fusion protein Ag16462
• Full Name: sestrin 1
• Calculated Molecular Weight: 551 aa, 64 kDa
• Observed Molecular Weight: 66-68 kDa
• GenBank Accession Number: BC112036
• Gene Symbol: SESN1
• Gene ID (NCBI): 27244
• RRID: AB_10793724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).

Publications

Species	Application	Title
mouse	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
mouse	WB	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
		Diabetes; PMID: 25377878; Authors - Rongya Tao
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner; Authors - Sujuan Liu
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
rat	WB	Eur J Neurosci; Overexpression of SESN1 improves mitochondrial damage and mitophagy, a potential therapeutic strategy for cognitive dysfunction after anaesthesia; Authors - Li Sun