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- KD/KO Validated
SESN1 Polyclonal antibody
SESN1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21668-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human skeletal muscle tissue, HEK-293 cells, mouse liver tissue, human testis tissue, HeLa cells, K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 18 publications below |
IHC | See 2 publications below |
Product Information
21668-1-AP targets SESN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SESN1 fusion protein Ag16462 |
Full Name | sestrin 1 |
Calculated Molecular Weight | 551 aa, 64 kDa |
Observed Molecular Weight | 66-68 kDa |
GenBank Accession Number | BC112036 |
Gene Symbol | SESN1 |
Gene ID (NCBI) | 27244 |
RRID | AB_10793724 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).
Protocols
Product Specific Protocols | |
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WB protocol for SESN1 antibody 21668-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine. | ||
Biochim Biophys Acta Mol Basis Dis Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner | ||
Osteoarthritis Cartilage Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism. | ||
Eur J Neurosci Overexpression of SESN1 improves mitochondrial damage and mitophagy, a potential therapeutic strategy for cognitive dysfunction after anaesthesia |