WDR24 Polyclonal antibody

• Featured Product
• KD/KO Validated

WDR24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 20778-1-AP

Synonyms

WD repeat-containing protein 24, JFP7, GATOR2 complex protein WDR24, EC:2.3.2.27, C16orf21

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Validation Data Gallery

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  WB analysis of HEK-293 using 20778-1-AP

  WB result of WDR24 antibody (20778-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.

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  WB analysis using 20778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20778-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20778-1-AP

  IP result of anti-WDR24 (IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.

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  IHC staining of human colon cancer using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20778-1-AP

  Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20778-1-AP

  Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IF: See 2 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below

Product Information

20778-1-AP targets WDR24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: WDR24 fusion protein Ag14774
• Full Name: WD repeat domain 24
• Calculated Molecular Weight: 920 aa, 102 kDa
• Observed Molecular Weight: 102 kDa, 88 kDa
• GenBank Accession Number: BC008025
• Gene Symbol: WDR24
• Gene ID (NCBI): 84219
• RRID: AB_10696183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

Publications

Species	Application	Title
	WB,IP	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
human	IP,WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long
human	WB,IP	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
mouse	WB,CoIP	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
human	WB	Nature; SZT2 dictates GATOR control of mTORC1 signalling.; Authors - Min Peng ; KO Validated
human	WB	Nat Commun; Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation; Authors - Xing Chen

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