WB result of WDR24 antibody (20778-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.
WB analysis using 20778-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20778-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 20778-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 20778-1-AP
IP result of anti-WDR24 (IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.
IP result of anti-WDR24 (IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.
IHC staining of human colon cancer using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 10x lens).
Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 20778-1-AP
Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human colon cancer tissue, human colon tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Hela cells, HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)
WB result of WDR24 antibody (20778-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.
WB analysis using 20778-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20778-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 20778-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon using 20778-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HeLa using 20778-1-AP
IP result of anti-WDR24 (IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.
IF/ICC Figures
IF Staining of HeLa using 20778-1-AP
Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HepG2 using 20778-1-AP
Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
WDR24 Polyclonal antibody
Catalog Number
20778-1-AP
Citations
25
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
