Rac1 Polyklonaler Antikörper

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• KD/KO Validated

Rac1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

78

Kat-Nr. : 24072-1-AP

Synonyme

MIG5, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

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Galerie der Validierungsdaten

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  WB analysis using 24072-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24072-1-AP (Rac1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 24072-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 24072-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP (RAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24072-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 24072-1-AP (Rac1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 72 publications below
• IHC: See 7 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Produktinformation

24072-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA Rac1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rac1 fusion protein Ag17779
• Vollständiger Name: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC004247
• Gene symbol: RAC1
• Gene ID (NCBI): 5879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
rat	WB,coIP	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human,mouse	IHC,WB	Cancer Res; CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder.; Authors - Xin Chen
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	WB,IF	Cell Mol Immunol; DOCK2 regulates antifungal immunity by regulating RAC GTPase activity.; Authors - Xiaojian Ma

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