RAC2 Monoclonal antibody

• Featured Product
• KD/KO Validated

RAC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, coIP, ELISA

Conjugate

Unconjugated

Publications

14

CloneNo.

4C2H6

Cat no : 60077-1-Ig

Synonyms

Ras-related C3 botulinum toxin substrate 2, p21-Rac2, p21 Rac2, HSPC022, Gx

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Validation Data Gallery

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  WB analysis using 60077-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60077-1-Ig (RAC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60077-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60077-1-Ig (RAC2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Product Information

60077-1-Ig targets RAC2 in WB, IHC, IF, coIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAC2 fusion protein Ag1202
• Full Name: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001485
• Gene Symbol: RAC2
• Gene ID (NCBI): 5880
• RRID: AB_2253445
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Publications

Species	Application	Title
rat	WB,coIP	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
mouse	WB	J Exp Med; Chemokine-coupled β2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.; Authors - Alan R Morrison ; KO Validated
mouse	WB	Prog Neurobiol; A γ-adducin cleavage fragment induces neurite deficits and synaptic dysfunction in Alzheimer's disease.; Authors - Min Xiong
human	WB	Cell Mol Immunol; DOCK2 regulates antifungal immunity by regulating RAC GTPase activity.; Authors - Xiaojian Ma
mouse	WB	Sci Signal; Rac signal adaptation controls neutrophil mobilization from the bone marrow.; Authors - Carlo Cosimo Campa
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo