RAC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10735-1-AP

Synonyms

EN 7, Gx, HSPC022, p21 Rac2, RAC2, Small G protein

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Validation Data Gallery

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  WB analysis using 10735-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10735-1-AP (RAC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 10735-1-AP

  IP result of anti-RAC2 (IP:10735-1-AP, 3ug; Detection:10735-1-AP 1:300) with HL-60 cells lysate 4000ug.

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  IHC staining of human tonsillitis using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10735-1-AP (RAC2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10735-1-AP (RAC2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10735-1-AP (RAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10735-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10735-1-AP (RAC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HL-60 cells, mouse spleen tissue, rat spleen tissue
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human tonsillitis tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

10735-1-AP targets RAC2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAC2 fusion protein Ag1202
• Full Name: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC001485
• Gene Symbol: RAC2
• Gene ID (NCBI): 5880
• RRID: AB_2176127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome.

Publications

Species	Application	Title
mouse	WB	Nat Genet; In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progression.; Authors - Meacham Corbin E CE ; KD Validated
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Front Mol Neurosci; Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.; Authors - Kai Zhang
human	WB	Oncol Rep; Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer.; Authors - Hao Bo
human	WB	Biochem Biophys Res Commun; Hsa_circ_001659 serves as a novel diagnostic and prognostic biomarker for colorectal cancer.; Authors - Baoyu He
human	WB	Tumour Biol; AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.; Authors - Zhaoyang Zeng