RAB7A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB7A Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

44

Rezensionen (6)

Kat-Nr. : 55469-1-AP

Synonyme

PRO2706, RAB7, RAB7A, Ras related protein Rab 7a

---

Galerie der Validierungsdaten

• 

  WB analysis using 55469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55469-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55469-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55469-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55469-1-AP (RAB7A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 55469-1-AP

  IP result of anti-RAB7A (IP:55469-1-AP, 4ug; Detection:55469-1-AP 1:1000) with SH-SY5Y cells lysate 3360 ug.

• 

  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55469-1-AP (RAB7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 55469-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55469-1-AP (RAB7A Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of A431 using 55469-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB7A (55469-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L-929-Zellen, A431-Zellen, C6-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe, RAW 264.7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Magengewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 31 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

55469-1-AP bindet in WB, IP, IHC, FC, ELISA RAB7A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB7A, member RAS oncogene family
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_004637
• Gene symbol: RAB7A
• Gene ID (NCBI): 7879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Publikationen

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
Zebrafish,monkey		Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
		Nat Commun; CHML promotes liver cancer metastasis by facilitating Rab14 recycle.; Authors - Tian-Wei Chen
human	WB	Autophagy; Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress; Authors - Jia-min Yan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Sophy (Verified Customer) (03-07-2023)

The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSCLC cells

FH

Malgorzata (Verified Customer) (08-17-2022)

The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: THP-1

RAB7A Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:55469-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

• Applications: Western Blot, Immunohistochemistry,
• Cell Tissue Type: HEKs

FH

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 Lysate

RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)

Verwandte Produkte

RILP Polyclonal antibody

13574-1-AP

RAB5A Polyclonal antibody

11947-1-AP

RAB9A Polyclonal antibody

11420-1-AP

RAB27A Polyclonal antibody

17817-1-AP