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  • KD/KO Validated

RAB35 Polyklonaler Antikörper

RAB35 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11329-2-AP

Synonyme

Ras-related protein Rab-1C, Ras related protein Rab 35, Ras related protein Rab 1C, RAB1C, GTP-binding protein RAY



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, A375-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe, RAW 264.7-Zellen
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Hirngewebe, humanes Lebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCU-87 MG-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11329-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA RAB35 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Hund, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RAB35 fusion protein Ag1872
Vollständiger Name RAB35, member RAS oncogene family
Berechnetes Molekulargewicht 201 aa, 23 kDa
Beobachtetes Molekulargewicht 23 kDa
GenBank-ZugangsnummerBC015931
Gene symbol RAB35
Gene ID (NCBI) 11021
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB35 is a member of the small GTPase superfamily. Like the other Rab proteins, RAB35 has 2 consecutive cysteine residues at its C terminus, and this region is thought to be involved in membrane association. Rab35 is found on the cell surface and endosomes, where it has been proposed to play a role in recycling of the transferrin and other receptors.

Protokolle

Produktspezifische Protokolle
WB protocol for RAB35 antibody 11329-2-APProtokoll herunterladen
IHC protocol for RAB35 antibody 11329-2-APProtokoll herunterladen
IF protocol for RAB35 antibody 11329-2-APProtokoll herunterladen
IP protocol for RAB35 antibody 11329-2-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Science

Identification of an oncogenic RAB protein.

Authors - Douglas B Wheeler
  • KD Validated
humanWB

Cell Metab

Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.

Authors - Chaitali Mukherjee
humanWB

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy
humanWB

Nat Cell Biol

Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1.

Authors - Alexander Wallroth
mouseWB,IF

Sci Adv

LRRK2 mediates tubulation and vesicle sorting from lysosomes.

Authors - Luis Bonet-Ponce
human,mouseWB,IHC,IF,CoIP

Nat Commun

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Authors - Corallino Salvatore
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Xin (Verified Customer) (03-15-2022)

A clean antibody to probe Rab35 with the right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human postmortem brain lysate
RAB35 Antibody Western Blot validation (1:2000 dilution) in human postmortem brain lysate (Cat no:11329-2-AP)
FH

Tom (Verified Customer) (02-24-2021)

HEK293T whole cell lysate probed with Rab35 antibody overnight at 4 degrees. Membrane was blocked in 5% BSA for one hour prior to incubation with antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
RAB35 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11329-2-AP)
FH

Mayur (Verified Customer) (01-14-2019)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain tissue (Cortex)
RAB35 Antibody Western Blot, validation (1:1000 dilution) in Mouse brain tissue (Cortex) (Cat no:11329-2-AP)