PRKCDBP Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PRKCDBP Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 16250-1-AP

Synonyme

Cavin 3, HSRBC, PRKCDBP, SRBC

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Galerie der Validierungsdaten

• 

  WB analysis of mouse lung using 16250-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16250-1-AP

  IP Result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:600) with mouse lung tissue lysate 2800ug.

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  IHC staining of human lung cancer using 16250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16250-1-AP (PRKCDBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 16250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 16250-1-AP (PRKCDBP antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 16250-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

16250-1-AP bindet in WB, IP, IHC, IF, ELISA PRKCDBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRKCDBP fusion protein Ag9209
• Vollständiger Name: protein kinase C, delta binding protein
• Berechnetes Molekulargewicht: 261 aa, 28 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC011585
• Gene symbol: PRKCDBP
• Gene ID (NCBI): 112464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

Publikationen

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
mouse	WB	PLoS Biol; Endocytic crosstalk: cavins, caveolins, and caveolae regulate clathrin-independent endocytosis.; Authors - Natasha Chaudhary
human	WB,IF	Nat Commun; Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.; Authors - Kerrie-Ann McMahon ; KD Validated
human	WB,IF,IP	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
mouse	WB, IF	Elife; Cavin-3 dictates the balance between ERK and Akt signaling.; Authors - Victor J Hernandez
mouse	WB	Elife; Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.; Authors - Eric Seemann ; KO Validated

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