Validation Data Gallery
|Positive WB detected in||COLO 320 cells, Jurkat cells, multi-cells, K-562 cells, HeLa cells, HEK-293 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10306-1-AP targets B23/NPM1 in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||B23/NPM1 fusion protein Ag0286|
|Full Name||nucleophosmin (nucleolar phosphoprotein B23, numatrin)|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC002398|
|Gene ID (NCBI)||4869|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
|Product Specific Protocols|
|WB protocol for B23/NPM1 antibody 10306-1-AP||Download protocol|
|IHC protocol for B23/NPM1 antibody 10306-1-AP||Download protocol|
|IF protocol for B23/NPM1 antibody 10306-1-AP||Download protocol|
|IP protocol for B23/NPM1 antibody 10306-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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