- Featured Product
- KD/KO Validated
AMPK Alpha Polyklonaler Antikörper
AMPK Alpha Polyklonal Antikörper für WB, IP, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (5)
Anwendung
WB, IHC, IF, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10929-2-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
| Erfolgreiche Detektion in IHC | humanes Prostatakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 12 publications below |
| WB | See 212 publications below |
| IHC | See 5 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Produktinformation
10929-2-AP bindet in WB, IHC, IF, IP, ELISA AMPK Alpha und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Fisch, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch, Ziege |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | AMPK Alpha fusion protein Ag1367 |
| Vollständiger Name | protein kinase, AMP-activated, alpha 1 catalytic subunit |
| Berechnetes Molekulargewicht | 63 kDa |
| Beobachtetes Molekulargewicht | 64 kDa |
| GenBank-Zugangsnummer | BC012622 |
| Gene symbol | AMPK Alpha 1 |
| Gene ID (NCBI) | 5562 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.10929-2-AP can recognize both AMPK Alpha 1 and AMPK Alpha 2.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladen |
| IHC protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladen |
| IP protocol for AMPK Alpha antibody 10929-2-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Autophagy The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.
| ||
Autophagy A plant nonenveloped double-stranded RNA virus activates and co-opts BNIP3-mediated mitophagy to promote persistent infection in its insect vector. | ||
J Hazard Mater Mitigation of honokiol on fluoride-induced mitochondrial oxidative stress, mitochondrial dysfunction, and cognitive deficits through activating AMPK/PGC-1α/Sirt3. | ||
J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Charlotte (Verified Customer) (08-15-2024) | Done in milk but got quite some background. I tried in seablock but not sure it helps. Otherwise nice antibody with one intense band at the expected MW
 |
FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
|
FH Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue
|
FH LUNFENG (Verified Customer) (01-27-2020) | GOOD
|
FH Jing (Verified Customer) (01-27-2020) | tested in mouse heart lysate, with clear single band around 60 kd.
|
FH Jie (Verified Customer) (01-27-2020) | showed a single band at the correct size with mouse heart tissue section
|
FH Yan (Verified Customer) (01-03-2020) | We tested this antibody in mouse heart tissue extract. It worked very well and showed a single band around 60kDa.
|
