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PPP1R15B Polyklonaler Antikörper

PPP1R15B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14634-1-AP

Synonyme

CREP, PPP1R15B



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA2780-Zellen, HEK-293-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, SGC-7901-Zellen, SKOV-3-Zellen
Erfolgreiche IPMCF-7-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe, Maus-Cerebellum-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFSH-SY5Y-Zellen, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14634-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA PPP1R15B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPP1R15B fusion protein Ag6213
Vollständiger Name protein phosphatase 1, regulatory (inhibitor) subunit 15B
Berechnetes Molekulargewicht 79 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC065280
Gene symbol PPP1R15B
Gene ID (NCBI) 84919
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

Protokolle

Produktspezifische Protokolle
WB protocol for PPP1R15B antibody 14634-1-APProtokoll herunterladen
IHC protocol for PPP1R15B antibody 14634-1-APProtokoll herunterladen
IF protocol for PPP1R15B antibody 14634-1-APProtokoll herunterladen
IP protocol for PPP1R15B antibody 14634-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell

Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.

Authors - Agnieszka Krzyzosiak
mouseWB

Neuron

Excitatory neuron-specific suppression of the integrated stress response contributes to autism-related phenotypes in fragile X syndrome

Authors - Mehdi Hooshmandi
mouseWB

Hepatology

PMID: 29698569

Authors - Xu Xu
  • KO Validated
humanWB,IF

Sci Adv

CReP mediates selective translation initiation at the endoplasmic reticulum.

Authors - Jonathan P Kastan
mouseWB

Nat Commun

Integration of Hippo signalling and the unfolded protein response to restrain liver overgrowth and tumorigenesis.

Authors - Hongtan Wu
mouseWB

Proc Natl Acad Sci U S A

An inhibitor of HIV-1 protease modulates constitutive eIF2α dephosphorylation to trigger a specific integrated stress response.

Authors - Aude De Gassart

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tatyana (Verified Customer) (03-11-2023)

Detects a major band ~100 kDa and shows upregulation in stressed cells (MG132 treatment). WB: 4% milk in TBST and semi-dry transfer; detects a double band at ~55kDa. Also was tested in IHC in mouse CNS tissue and in ICC in human cells; the signal appears specific in both cases (not shown).

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, SH-SY5Y cells; mouse, brain
PPP1R15B Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in Human, SH-SY5Y cells; mouse, brain (Cat no:14634-1-AP)
FH

Will (Verified Customer) (10-24-2019)

good antibody, recommended by a colleague, would order again

  • Applications: Western Blot,
  • Cell Tissue Type: hepatocytes