HOMEZ Polyclonal antibody

HOMEZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23965-1-AP

Synonyms

HOMEZ, KIAA1443

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Validation Data Gallery

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  WB analysis using 23965-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23965-1-AP (HOMEZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23965-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23965-1-AP (HOMEZ Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 23965-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HOMEZ antibody (23965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23965-1-AP targets HOMEZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HOMEZ fusion protein Ag21090
• Full Name: homeobox and leucine zipper encoding
• Calculated Molecular Weight: 525 aa, 59 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC130392
• Gene Symbol: HOMEZ
• Gene ID (NCBI): 57594
• RRID: AB_2879378
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Transl Androl Urol; Homeodomain protein HOMEZ is dispensable for male fertility in mice.; Authors - Qiuling Yue