PHOX2B Polyklonaler Antikörper

PHOX2B Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 25276-1-AP

Synonyme

NBPhox, Neuroblastoma Phox, paired like homeobox 2b, PHOX2B, PHOX2B homeodomain protein, PMX2B

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Galerie der Validierungsdaten

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  WB analysis of Neuro-2a using 25276-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 25276-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 25276-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of neuroblastoma using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of neuroblastoma using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 25276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of Neuro-2a using 25276-1-AP

  Immunofluorescent analysis of Neuro-2a cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 25276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Neuroblastomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Neuro-2a-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

25276-1-AP bindet in WB, IHC, IF, ELISA PHOX2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHOX2B fusion protein Ag13196
• Vollständiger Name: paired-like homeobox 2b
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC017199
• Gene symbol: PHOX2B
• Gene ID (NCBI): 8929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.

Publikationen

Species	Application	Title
rat	WB	Stem Cells Transl Med; Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta.; Authors - Ling Ma
mouse	IHC	Mol Metab; Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance.; Authors - Ceren Ozek
human	IF	Sci Rep; Generating trunk neural crest from human pluripotent stem cells.; Authors - Miller Huang
human	IF	Sci Rep; Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.; Authors - Yuzo Takayama
human	IHC	Int J Mol Sci; Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis; Authors - Yuzo Takayama

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