NeuN Polyclonal antibody

NeuN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 23060-1-AP

Synonyms

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Validation Data Gallery

• 

  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 23060-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23060-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23060-1-AP (NeuN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 23060-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23060-1-AP (NeuN Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, 3T3-L1 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 8 publications below

Product Information

The immunogen of 23060-1-AP is NeuN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, hamster, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NeuN fusion protein Ag19347
• Full Name: hexaribonucleotide binding protein 3
• Observed Molecular Weight: 38-50 kDa
• GenBank Accession Number: BC093713
• Gene Symbol: NeuN
• Gene ID (NCBI): 146713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuN, also named as FOX3 and RBFOX3, is neuronal-specific nuclear protein with MW 38-50kd(~48kd). It is one of a family of 3 mammalian Fox homologues. FOX3(Or NeuN) functions in RNA-binding protein that regulates alternative splicing events.

Publications

Species	Application	Title
goat	IF	Cell Cycle; Effects of the histone acetylase inhibitor C646 on growth and differentiation of adipose-derived stem cells.; Authors - Zhimin Wang
hamster	IHC	Neurotherapeutics; PMID: 28083805; Authors - Syed Zahid Ali Shah
rat	IF	Exp Cell Res; CEBPD aggravates apoptosis and oxidative stress of neuron after ischemic stroke by Nrf2/HO-1 pathway; Authors - Nan Chen
rat	WB,IF	Neurochem Int; Tert-butylhydroquinone post-treatment attenuates neonatal hypoxic-ischemic brain damage in rats.; Authors - Juan Zhang
mouse	IF	J Stroke Cerebrovasc Dis; An Increase of Sigma-1 Receptor in the Penumbra Neuron after Acute Ischemic Stroke.; Authors - Xiaotian Zhang
human	IHC	Pediatr Surg Int; Pathological changes of interstitial cells of Cajal and ganglion cells in the segment of resected bowel in Hirschsprung's disease.; Authors - Xuyong Chen