PDGFR alpha Monoklonaler Antikörper

PDGFR alpha Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2C11B8

Rezensionen (1)

Kat-Nr. : 60045-1-Ig

Synonyme

CD140A, CD140a antigen, PDGF R alpha, PDGF receptor alpha, PDGFR2, PDGFRA, PDGFRα, PDGFR-α, Rhe PDGFRA

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Galerie der Validierungsdaten

• 

  WB analysis of THP-1 using 60045-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 60045-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 60045-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 60045-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of THP-1 using 60045-1-Ig

  1X10^6 THP-1 cells were stained with 0.20ug PDGFRA antibody (60045-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HT-1080.Zellen, HUVEC-Zellen, U-251-Zellen
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 3 publications below

Produktinformation

60045-1-Ig bindet in WB, IF, FC, ELISA PDGFR alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDGFR alpha fusion protein Ag1385
• Vollständiger Name: platelet-derived growth factor receptor, alpha polypeptide
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa, 140-160 kDa
• GenBank-Zugangsnummer: BC015186
• Gene symbol: PDGFRA
• Gene ID (NCBI): 5156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Publikationen

Species	Application	Title
human	WB,IF	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition; Authors - Tiffany L. Dill
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.; Authors - Tiffany L Dill
mouse	WB	Metabolism; The vitamin D receptor regulates miR-140-5p and targets the MAPK pathway in bone development.; Authors - Wenting Luo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (11-25-2021)

HEK293 lysate - some non-specific bands below real band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

PDGFR alpha Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:60045-1-Ig)

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