B23/NPM1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

B23/NPM1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

28

CloneNo.

4F12A3

Rezensionen (1)

Kat-Nr. : 60096-1-Ig

Synonyme

B23, B23/NPM1, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

---

Galerie der Validierungsdaten

• 

  WB analysis using 60096-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 60096-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60096-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60096-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60096-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60096-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60096-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60096-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60096-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60096-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 60096-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 14 publications below
• IP: See 4 publications below

Produktinformation

60096-1-Ig bindet in WB, IP, IHC, IF, ELISA B23/NPM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: B23/NPM1 fusion protein Ag7415
• Vollständiger Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC002398
• Gene symbol: NPM1
• Gene ID (NCBI): 4869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.; Authors - Pengbo Cao
human	WB,IF	Nat Commun; A redox mechanism underlying nucleolar stress sensing by nucleophosmin.; Authors - Kai Yang ; KD Validated
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	Aging Cell; Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.; Authors - Patricia Gomez-Suaga
human	WB,IF	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB,IF	Hum Mol Genet; Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.; Authors - Zhouteng Tao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tatyana (Verified Customer) (12-04-2023)

Was used for nucleolar staining, very strong specific signal

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / HeLa cells

B23/NPM1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:60096-1-Ig)

Verwandte Produkte

NCL Polyclonal antibody

10556-1-AP

ERp57/ERp60 Polyclonal antibody

15967-1-AP

B23/NPM1 Polyclonal antibody

10306-1-AP

HNRNPK Polyclonal antibody

11426-1-AP