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- KD/KO Validated
HNRNPK Polyclonal antibody
HNRNPK Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 11426-1-AP
|Positive WB detected in||MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11426-1-AP targets HNRNPK in WB, RIP, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, deer, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HNRNPK fusion protein Ag1976|
|Full Name||heterogeneous nuclear ribonucleoprotein K|
|Calculated molecular weight||463 aa, 51 kDa|
|Observed molecular weight||55-65 kDa|
|GenBank accession number||BC014980|
|Gene ID (NCBI)||3190|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability
|Product Specific Protocols|
|WB protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|IHC protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|IF protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|IP protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|FC protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
DEBJIT (Verified Customer) (09-17-2020)
WB: 1:2000IP: 2ug/mg cell lysate