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  • KD/KO Validated

HNRNPK Polyclonal antibody

HNRNPK Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, FC, chIP, ELISA

Conjugate

Unconjugated

Cat no : 11426-1-AP

Synonyms

CSBP, hnRNP K, HNRNPK, HNRPK, TUNP



Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11426-1-AP targets HNRNPK in WB, RIP, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, deer, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPK fusion protein Ag1976
Full Name heterogeneous nuclear ribonucleoprotein K
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC014980
Gene symbol HNRNPK
Gene ID (NCBI) 3190
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability

Protocols

Product Specific Protocols
WB protocol for HNRNPK antibody 11426-1-APDownload protocol
IHC protocol for HNRNPK antibody 11426-1-APDownload protocol
IF protocol for HNRNPK antibody 11426-1-APDownload protocol
IP protocol for HNRNPK antibody 11426-1-APDownload protocol
FC protocol for HNRNPK antibody 11426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Authors - Wenqi Xu
humanWB

Cell

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Authors - Lei Sun
humanWB

Cell Metab

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

Authors - Mi Kyung Park
WB

Nat Commun

Profiling PRMT methylome reveals roles of hnRNPA1 arginine methylation in RNA splicing and cell growth.

Authors - Wen-Juan Li
  • KD Validated

Nat Commun

PRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments.

Authors - Chen-Yu Wang

Nucleic Acids Res

Capturing RNA-protein interaction via CRUIS.

Authors - Ziheng Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

DEBJIT (Verified Customer) (09-17-2020)

WB: 1:2000IP: 2ug/mg cell lysate

  • Applications: Western Blot, Immunoprecipitation
  • Cell Tissue Type: Endothelial cells